Time-Resolved Fluorometry Based Sandwich Hybridisation Assay for HLA-DQA1 Typing
نویسندگان
چکیده
منابع مشابه
Time-Resolved Fluorometry Based Sandwich Hybridisation Assay for HLA-DQA1 Typing
A microtitration plate based time-resolved fluorescence (TRF) hybridisation assay was developed for HLA typing utilising biotinylated sequence-specific catching probes and europium (Eu) labelled gene locus-specific detection probe to allow time-resolved fluorometer reading of the reaction. In an application for HLA-DQA typing a 228 base pair long region of the polymorphic exon 2 of DQA1 gene wa...
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We report microtiter well-based sandwich-type DNA hybridization assays using enzyme amplified time-resolved fluorometry of Tb3+ chelates. The target DNA was hybridized with two adjacent and non-overlapping oligonucleotide probes, one oligonucleotide serving as the capture probe and the other as the detection probe. Two ligand-specific binding protein pairs were used alternately for capture of t...
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In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a smal...
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BACKGROUND Preactivated solid surfaces provide new possibilities for multiple consecutive reactions in a microtiter plate format. In this study, a combination of PCR and subsequent hybridization in the same microtiter well was applied for the detection of HLA-B27 alleles. METHODS A multiplex solid-phase PCR to amplify the HLA-B27 alleles together with beta-actin as an amplification control ge...
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ژورنال
عنوان ژورنال: Disease Markers
سال: 1998
ISSN: 0278-0240,1875-8630
DOI: 10.1155/1998/350145